枯草芽孢杆菌芽孢对猪扁桃体内树突状细胞的影响

doi:10.11843/j.issn.0366-6964.2015.05.023

Abstract

Abstract:

Pigs were administrated by intranasal with Bacillus subtilis spores.Three hours later，the numbers of dendritic cells (DC) in tonsils were studied.Ten crossed-bred（Duroc×Landrace×Yorkshine） pigs aged 2 months were randomly divided into two groups，Nasal administration with PBS or Bacillus subtilis spores respectively.Three hours later，the tube tonsils and soft palate tonsils were sampled and prepared tissue slices.Three anti-porcine dendritic cells antibodies，MHCII，CD11b and CD16 were used to examined the changes of tonsil DC in porcine tonsils.Our results showed that：after intranasal administration of Bacillus subtilis spores，the numbers of CD11b⁺CD16⁺ DCs and CD11b⁺MHCII⁺ DCs in tube tonsils have increased significantly（P<0.05），approximately two or three times higher than normal controls；the numbers of CD11b⁺CD16⁺ DCs and CD11b⁺MHCII⁺ DCs in soft palate tonsils have increased significantly also（P<0.05）.Moreover，the numbers of CD11b ⁺MHCII⁺ DCs were higher than CD11b⁺CD16⁺ DCs，but the difference was not significant.These results suggested that intranasal administration with Bacillus subtilis spores could induce accumulation of dendritic cells in tonsils and promote dendritic cell maturation，therefore effectively improve the ability of tonsils to resist pathogens invasion.

CLC Number:

S852.1

SHEN Yu-meng，YANG Qian. Effects of Intranasal Administration with Bacillus subtilis Spores on the Dendritic Cells in Porcine Tonsils[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(5): 849-854.

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Effects of Intranasal Administration with Bacillus subtilis Spores on the Dendritic Cells in Porcine Tonsils

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